Preoperative phlebotomy removed 9.3 +/- A 2.9 mL/kg of blood, and 5.6 +/- A 5.1 mL/kg of crystalloid was administered intraoperatively. Within 30 min learn more after phlebotomy, 23 patients had a MAP decrease of 20 % or more from baseline. This fall in MAP coincided with a decrease in rSO(2) of 20 or more at 2 of 114 measured points. Initially, rSO(2) decreased from 74 +/- A 9 to 68 +/- A 10 but thereafter remained constant. On five occasions, rSO(2) decreased 20 or more from baseline, but no patient’s NIRS value was less than 45. A decrease in rSO(2) occurred more commonly in younger patients and those who had a larger volume of blood removed. Preoperative phlebotomy without significant

volume replacement can be performed safely before CPB. Volume replacement may be more appropriately guided by rSO(2) than by hemodynamic variables.”
“Spermatozoa deliver more than the paternal genome into the oocyte; they also carry remnant messenger RNA from spermatogenesis. The RNA profiles of SNX-5422 supplier spermatozoa from high-fertility and a low-fertility Holstein bulls were analysed using Affymetrix bovine genechips. A total of 415 transcripts

out of approximately 24,000 were differentially detected in spermatozoa collected from both bulls (fold change >= 2.0; P < 0.01). These transcripts were associated with different cellular functions and biological processes. Spermatozoa from high-fertility bulls contained higher concentrations of transcripts for membrane and extracellular space protein locations, while spermatozoa from the low-fertility bulls were deficient of transcripts for transcriptional and translational factors. Quantitative real-time PCR was used on three low-fertility and four high-fertility bulls to validate the microarray data. Two highly represented transcripts

in the microarray analysis (protamine 1 and casein beta 2) were validated, as well as a third transcript (thrombospondin receptor CD36 molecule) that showed a lower concentration in low-fertility bulls. This study presents the global analysis of spermatozoa originating from bulls with opposite fertility. A1155463 These results provide some specific transcripts in spermatozoa that could be associated with bull fertility. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.”
“The diagnosis and management of prenatal tachyarrhythmias is well established; however, the postnatal course and outcomes are not. The purpose of our study was to review the natural history of patients with fetal tachycardia, determine the incidence of postnatal arrhythmias, and determine whether there are factors to predict which fetuses will develop postnatal arrhythmias. A retrospective chart review of patients with fetal tachyarrhythmias investigated at British Columbia Children’s and Women’s Hospitals between 1983 and 2010 was conducted. Sixty-nine mother-fetus pairs were eligible for the study.