31 1.31   1.31 1.21 1.31 1.31 1.31   Perfringolysin O CPF_0156 CPE0163   AC5_0210 CJD_0196 CPC_0186 AC3_0278 AC1_0175 “” pfoA 1.18 1.18   1.18 1.18 1.18 1.18 1.18   Reg. RNA CPF_0925 CPE0920   * CJD_1073 * AC3_1102 AC1_1131 “” virU 1.20 1.20   1.26 1.26 1.20 1.20 1.20   hypothetical CPF_1074   CPR_0937 **     ** ** [8]   0.88   1.11 1.03     1.03 1.03   hypothetical CPF_0461   CPR_0762         AC1_0537 “”   1.28   1.38         1.28   hypothetical       AC5_0209   CPC_0185               1.18   1.18       Reg. RNA   CPE0845           AC1_0990 [7] virT   1.2977           1.29   Predicted VirR regulons, only genes present in at least two genomes

are shown. Numbers below each gene YM155 solubility dmso name correspond to the score Saracatinib concentration calculated as described in Methods (on a maximum attainable score of 1.52). As described in the text, most of the known VirR targets belongs to this group. * no open reading frame identified in this region but DNA sequence identical to CPE0920; ** no open reading frame identified in this region but DNA sequence identical to CPF_1074, †: draft genomes. Table 3 Strain specific VirR targets Product Gene Score Dist.

Strain 2-keto-3-deoxygluconate kinase AC3_0259 1.26 124 JGS1987† hypothetical protein AC3_0622 AC3_0622 1.16 70 JGS1987† hypothetical protein AC3_A0724 AC3_A0724 1.04 393 JGS1987† hypothetical protein AC3_A0725 AC3_A0725 1.04 119 JGS1987† conserved hypothetical protein AC3_A0081 1.11 180 JGS1987† resolvase/recombinase AC3_0180 1.15 264 JGS1987† put. lipid A export ATP-binding/permease (MsbA) AC3_0181 1.15 124 JGS1987† hypothetical protein AC3_A0587 AC3_A0587 1.34 227 JGS1987† hypothetical protein AC3_0277 AC3_0277 1.18 112 JGS1987† hypothetical protein

AC3_A0194 AC3_A0194 1.25 284 JGS1987† hypothetical protein AC1_A0478 Fossariinae AC1_A0478 0.80 75 ATCC 3626† hypothetical protein AC5_A0236 AC5_A0236 1.04 110 F4969† put. metal-dependent hydrolase CPR_1028 1.34 499 SM101 hypothetical protein CJD_0545 CJD_0545 0.95 153 JGS1721† hypothetical protein CJD_1387 CJD_1387 1.30 75 JGS1721† Genes identified as VirR targets that are present in a single strain. Strain JGS1987 suggests an expansion of the VirR regulon. †: draft genomes. One target only appeared to be conserved in all tested strains, corresponding to the α-clostripain gene. Four genes were shown to be conserved in all strains but SM101. Interestingly, strain SM101 appeared to have the www.selleckchem.com/products/blz945.html lowest degree of conservation of VirR targets. A search for the corresponding gene sequences in the genome confirmed that they are absent, in agreement with a previous comparative analysis that showed the absence of several virulence factors and toxins and the presence of specific repertoire of genes encoding bacteriocins [8]. On the converse, missing genes in draft genomes cannot be considered as surely absent.