4 98.8 99.5 99.5 lpl0803 A ORF 13 – 40.3 40.3 40.3 40.3 trans.c 100 98.2 98.2 96.6 41.8 40.3 40.3 lpg0765 ORF 12 100 98.6 98.7 98.6 98.6 – - – - – 98.7 98.6 trans.c lpg0766 ORF 11 100 96.6 96.6 96.6 96.6 93.2 93.2 93.7 93.7 93.1 96.6 96.6 96.6 lpg0767 ORF 10 100 96.2 96.2 96.2
96.2 96.6 97.1 98.9 98.9 97 95.6 96.2 96.2 lpg0768 ORF 9 100 30.6 30.6 30.6 30.6 98.4 99 99 99 98.9 99.4 30.6 30.6 lpg0769 Ruboxistaurin concentration ORF 8 100 31 31 31 31 97.9 97.4 98.4 98.4 97.4 100 31 31 lpg0770 ORF 7 100 90.6 90.6 90.6 90.6 32 31.9 31.9 31.9 99.8 99.9 90.6 90.6 lpg0771 ORF 6 100 38.8 38.7 38.7 38.7 38.8 99.1 100 100 38.8 38.6 99.1 38.7 lpg0772 (wzm) ORF 5 100 100 100 100 100 100 100 100 100 100 100 GW786034 price 100 100 lpg0773 (wzt) ORF 4 100 99 99.6 100 100 100 99.6 100 99.5 99 99.8 100 100 lpg0774 ORF 3 100 91.6 86.4 98.7 92.1 89 86.4 100 86.4 91.6 99.5 99.8 99.8 lpg0775 a 100 – 100 – - – - – - – - – lpg0776 b 100 – - 100 – - – - – - – - – lpg0777 (lag-1) 100 96.8 94.9 100 96.8 94.9 94.9 – 94.7† 96.8 – - – lpg0778 ORF 2 100 97.9 97.4 100 97.7 97.4 97.4 99.6 96.5 97.9 98.9 98.7 98.7 lpg0779 ORF 1 100 99.8 99.1 99.8 99.8 98.9 98.9 100 98.9 99.8
99.4 99.8 99.8 # Monoclonal antibody subgroup according to the ‘Dresden’ panel. * Determined by UPGMA clustering method based on multiple sequence alignment. A ORF 13 (lpg0764/lpg0764b/lpg0763) of Philadelphia 1 not displayed, ORF 13-A of strain Lens was used. a Partial selleck compound duplication of ORF 2 (lpg0778). b, c Transposase; transposase disrupted. † Lag-1 of Görlitz 6543 has no functional
start codon. Underlined numbers indicate different clusters of corresponding ORF (see also Figure 2). The highly conserved 15 kb region (ORF14 – ORF 28) is not completely shown and only reflected by WecA and GalE. A conserved region found in all serogroup 1 strains Within the conserved region several genes were found which are proposed to be involved in the biosynthesis of the highly acetylated core region which is composed of mannose, Arachidonate 15-lipoxygenase N-acetyl-glucosamine (GlcNAc), N-acetyl-quinovosamine (QuiNAc) and rhamnose residues [19]. A vast number of ORFs, more specifically ORF 21 through 25 and 28, were recently reported to facilitate the biosynthesis of the repetitive legionaminic acid residues of the O-antigen [18, 36]. The pyrodoxal-phosphate dependent aminotransferase (ORF 21), the acetyltransferase neuD (ORF 22) and a dehydratase (lpg0966) located outside of the locus are likely to synthesize the precursor molecule of legionaminic acid, UDP-N,N’-diacetylbacillosamine (UDP-Bac2Ac4Ac) [37].