5 ml at two sites. At day 28 animals were boosted with 100μg ml-1 protein per animal using incomplete Freund’s adjuvant. At day 56 a second booster injection identical to the first booster injection was performed and at day 69 the animals were bled to check for the antibody titre. Gel electrophoresis and Western blotting Protein samples diluted with 1:1 sample buffer (60 mM Tris–HCl, pH 6.8, 2% SDS, 10% glycerol, 0.025% bromophenol blue) were separated on 10% polyacrylamide – SDS gels. For Western blotting analysis, separated proteins were electrophoretically transferred onto a polyvinylidene fluoride membrane (PVDF, 0.2μm, BioRad). Protein bound PVDF membranes were blocked with 5% milk and incubated with polyclonal anti-FAAH EGFR inhibitor antibody
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