As cathepsin L prefers a hydrophobic residue at P2 and cathepsin B prefers an arginine at the same position ( Barrett et al., 1998), S. levis

cysteine proteinase is a cathepsin L-like proteinase. Its molecular mass, as determined by SDS-PAGE (37 kDa), is somewhat larger than and its optimal pH (6.0) is similar to known insect cathepsin L-like proteinases (see, for example, BGB324 Cristofoletti et al., 2005). The food ingested by insects generally passes through the foregut and is enclosed by the PM in the midgut, where it is digested first by enzymes that penetrate into the endoperitrophic space (inside the PM), then by enzymes acting on diffuse material in the ectoperitrophic space (between the PM and midgut epithelium) and finally on the midgut cell surface (Terra and Ferreira, 1994 and Terra and Ferreira, 2005).

The PM is a film that surrounds the food bolus in most insects and is formed by a network of chitin and proteins to which enzymes and other components associate. selleck screening library This structure shares with the ancestral gastrointestinal mucus the functions of protection against food abrasion and microorganisms, but also has specific functions in digestion associated to the compartmentalization of luminal contents (Terra, 2001 and Bolognesi et al., 2008). Occasionally, the film surrounding the food may have a gel consistency, forming a non-membranous structure known as peritrophic gel (PG) (Terra, 2001 and Terra and Ferreira, 2005). The presence of a conspicuous PM in S. levis was confirmed by dissection only in the middle and posterior regions of the midgut, whereas the observations of the present study indicate the occurrence of a PG in the anterior midgut. Enzyme assays in S. levis demonstrated that the initial digestion of starch must be carried out by amylase in the anterior and middle portions of the midgut, whereas final starch digestion

must be performed by maltase. Protein digestion starts under the action of a cathepsin L-like proteinase in the anterior and middle midgut, Exoribonuclease continues with trypsin in middle and posterior midgut and finishes on the surface of cells in the middle and posterior midgut by a membrane-bound aminopeptidase. Soluble trypsin and capthepsin L-like enzymes found associated with midgut tissue probably correspond to enzyme molecule entrapped in the cell glycocalyx, as shown in other beetles ( Ferreira et al., 1990). Membrane-bound trypsin has been described in other insect midguts and seems to be enzyme molecules en route to be secreted ( Terra and Ferreira, 1994 and Terra and Ferreira, 2005). The activities of amylase, cysteine proteinase and trypsin decrease throughout the contents of the midgut. This is what one would expect when there is a flux of fluid from the posterior midgut to the anterior midgut in the ectoperitrophic space, as described for most insects (for reviews, see Terra and Ferreira, 1994 and Terra and Ferreira, 2005).