Bayesian clustering of the ISSR data using STRUCTURE supported the presence of three Vadimezan nmr clusters among the isolates (Figure 2). Both Maximum parsimony (not shown) and NJ trees (Figure 3) were in agreement with the clusters defined by STRUCTURE. Although there was no significant bootstrap support for two of the clades on the NJ tree [1] and [3], support for clade 2 was 94%. Clade 1, composed exclusively of isolates from Europe, contained 27 of the 113 isolates. Sixteen isolates in this European clade were from Italy and 11 isolates were from Belgium or France. The type of line in Figure 3 indicates the cluster membership of each isolate on the NJ tree and illustrates the correlation

between AZD5582 clades and clusters. Bayesian clustering of the ISSR data also supported the existence of the European clade. (Figure 3) The European cluster 1, as revealed by STRUCTURE, contained 34 isolates while the European clade 1 (NJ

and MP algorithms) contained 27 of the same isolates. Many European isolates did not, however, Nutlin3a fall into the exclusively European cluster or clade. Figure 2 STRUCTURE grouping of A. terreus isolates. Inferred population structure of A. terreus isolates from STRUCTURE analysis of ISSR data. Each isolate is represented by a vertical bar partitioned into shaded segments representing the isolate’s estimated proportion of ancestry from each of three clusters defined by STRUCTURE. Figure 3 Neighbor joining tree from ISSR fingerprints of A. terreus isolates. Phylogenetic relationship

among A. terreus isolates inferred by ISSR fingerprints using the Neighbor joining algorithm. The tree is rooted with the outgroup Aspergillus fumigatus. Bootstrap values above 50% from 1000 iterations are noted on nodes. Lines indicate isolate affiliation with clusters defined by STRUCTURE. Filled and open circles and squares indicate geographic origin of isolates. A significant relationship existed between geography and cluster membership (X2 = 48.2, d.f. = 6, p < 0.001), driven primarily by cluster 1 being composed only of isolates from Europe, as well as cluster 2 accounting for the majority [12 of 19] of the sequence-confirmed Eastern U.S. A. terreus isolates (Figure 2). The Thiamet G patterns of cluster membership in the two US populations were similar to each other and quite different from the pattern shared by the two European populations (Figure 2). There were nine isolates in which the majority contribution from any cluster was less than 0.66, suggesting that these isolates did not consistently fall into any one cluster. These isolates were excluded from any single cluster due to their high levels of inferred admixture. Susceptibility testing to AMB Susceptibility testing to AMB for all the isolates analyzed in this investigation was available through a previous study summarized in Table 1 of Tortorano et al [12]. The isolates in each of the three clusters varied in mean MIC values (0.78, 1.29 and 0.86 mg/L for clusters 1, 2 and 3 respectively (Table 2).