Biochemical evaluation ical detection process of Wagner et al The analytical column implemented was a BIORAD ODS 5S reverse phase column . The detector possible was set at 0.9 mV versus Ag AgC1 reference electrode. Mobile phase was composed of seven mM phosphate 15 mM citrate buffer with five methanol and 25 50 mg of sodium octyl sulfate since the ion pair agent . The remaining half brains have been assayed for DOPAC, HVA, 5 HIAA and 5 HT in accordance on the LC EC method of Perry and Fuller . The column utilized was a C6 HiCHROM reversible column . The detector potential to the carbon paste electrode was set at 0.9 mV vs. Ag AgC1 reference electrode. The mobile phase was composed of 0.one M phosphate 0.05 M citrate buffer with 10 methanol . two.6. Statistical examination Behavioral information obtained in the PCPA five HTP experiment had been analyzed employing a 3 aspect, PCPA X 5 HTP x TRIALS, analysis of variance with repeated measures for the TRIALS factor. Significant major effects and interactions had been probed employing the submit hoc Newman Keuls test.
Behavioral data from animals taken care of using the 5 HT receptor antagonists have been analyzed making use of two element, DRUG x DOSE, examination of covariance with pretest scores for the behavioral measures as the covariates. Only comparisons of the certain drug treatment with its respective handle were examined dependant on the process for planned comparisons described by Winer . Biochemical data had been analyzed utilizing ANOVA. A priori planned comparisons of drug therapy results on brain monoamine and metabolite phosphatase inhibitor kinase inhibitor levels with all the ranges through the unique control groups had been analyzed . Animals were sacrificed by cervical dislocation and their brains instantly eliminated and rinsed in ice cold saline . Brains were dissected into two hemispheres, weighed and frozen till assayed . Half brains had been assayed for NE and DA using the substantial pressure liquid chromatography with electrochem 3. Final results three.one. Results of PCPA and or five HTP on aggressive behavior Fig. 1 illustrates that PCPA increased assault latency in the dose dependent method. The a hundred signifimg kg dose of five HTP was also successful in rising the latency to assault.
These drugs seemed to possess cumulative suppressive results on aggressive habits seeing that the latency scores with the treatment groups had been considerably longer on trial 3 than on trial one . The results of PCPA and 5 HTP on variety of attacks are presented in fig. two. The 100 mg kg dose Diabex of five HTP at the same time as the two doses of PCPA had been successful in suppressing the quantity of attacks emitted by postpartum mice. 3.2. Results of PCPA and or 5 HTP on locomotor exercise As could be witnessed in fig. 3, each doses of PCPA had exercise minimizing effects only on trial two . Only the 100 mg kg dose of five HTP reduced activity on trial 1 , although the two doses of five HTP reduced activity on trial 2 . three.three.