“”Comparative Analysis of Capsules of Sibutramine Acquired in Formal and Informal Market”. Sibutramine is an anorexigenic agent indicated for the treatment of obesity and body weight reduction, together with a food diet and physical exercises. In Brazil, it is found commercially in the pharmaceutical form of 10 and 15 mg capsules. Medication containing this agent can be purchased in Brazil nowadays in both the formal and informal market. Hence, the aim of this study is to do a comparative analysis of uniformity of mass and content between the reference, the generic, the similar, the homeopathic and the informally commercialised medicines, by using spectrophotometry
in the ultraviolet region. According AS1842856 Metabolism inhibitor to the results obtained, as to the average weight all the samples have fulfilled the specifications; however, in the uniformity of content test only the sample acquired in the informal market presented irregular concentration of the active substance.”
“An HPLC system combining a chemiluminescence detector was applied to estimate the singlet oxygen (O-1(2)) generation ability of di-sulfonic phthalocyanine zinc (ZnPcS2) isomers. As photosensitizers, ZnPcS2 produces O-1(2) in air-saturated solutions under photoirradiation. The latter reacts with methyl Cypridina luciferin analogue (MCLA) to
initiate chemiluminescence. This photoinduced chemiluminescence (PCL) of MCLA provides an easy method for evaluating the isomers’ O-1(2) generation ability during a simultaneous HPLC separation HDAC inhibitor procedure. The cis-isomers and trans-isomers of ZnPcS2 show different O-1(2) generation abilities, which are in accordance with differences
in their absorption spectra. Copyright (c) 2013 John Wiley & Sons, Ltd.”
“Glycosaminoglycans (GAGs) are ubiquitously present at the cell surface and in extracellular matrix, and crucial for matrix assembly, cell-cell and cell-matrix interactions. The supramolecular presentation of GAG chains, along with other matrix components, is likely to be functionally important but remains challenging to control and to characterize, both in vivo and in vitro. We present a method to create well-defined biomimetic surfaces that display GAGs, either alone or together with other cell ligands, in a background that suppresses non-specific binding. Through the design of the immobilization platform a streptavidin monolayer serves as a molecular Cell Cycle inhibitor breadboard for the attachment of various biotinylated ligands and a set of surface-sensitive in situ analysis techniques (including quartz crystal microbalance and spectroscopic ellipsometry), the biomimetic surfaces are tailor made with tight control on biomolecular orientation, surface density and lateral mobility. Analysing the interactions between a selected GAG (heparan sulphate, HS) and the HS-binding chemokine CXCL12 alpha (also called SDF-1 alpha), we demonstrate that these surfaces are versatile for biomolecular and cellular interaction studies.