ISME J 2010, 4:642–647.PubMedCrossRef 17. Giovannoni S: Oceans of bacteria. Nature 2004, 430:515–516.PubMedCrossRef 18. Bell T, Newman JA, Silverman BW, Turner SL, Lilley AK: The contribution of species richness and composition to bacterial services. Nature 2005, 536:1157–1160.CrossRef 19. Vasconcelos JT, Shaw LM, Lemon KA, Cole NA, Galyean ML: Effects of graded levels of sorghum wet distillers grains and degraded intake protein on performance and carcass characteristics of feedlot cattle fed steam-flaked corn-based diets. Prof Anim Sci 2007, 23:467–475. 20. Lozupone C, Knight R: Unifrac: a new CDK inhibitor phylogenetic method for comparing microbial
communities. Appl Environ Microbiol 2005, 71:8228–8235.PubMedCrossRef 21. Anderson MJ, Willis TJ: Canonical analysis of principal coordinates: a useful method of constrained ordination for ecology. Ecology 2003, 84:511–525.CrossRef 22. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger selleck screening library K, Bushman FD, Costello EK, Fierer N, Gonzalez Pena A, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko
T, Zaneveld J, Knight R: Qiime allows analysis of high-throughput community sequencing data. Nat Methods 2010, 7:3356.CrossRef 23. R Development Core Team: R: a language and environment for statistical computing. Vienna, Immune system Austria: R Foundation for Statistical Computing; 2011. ISBN ISBN 3–900051–07–0 24. Oksanen J, Guillaume Blanchet F, Kindt R, Legendre P, O’Hara RB, Cytoskeletal Signaling inhibitor Simpson GL, Solymos P, Stevens MMH, Wagner H: vegan: Community ecology package. 2011. 25. Roberts DW: labdsv: Ordination and multivariate analysis for ecology. 2010. Authors’ contributions MG and NAC designed the feeding trial which was
conducted by MG; WCR and NAC acquired the samples. SED, SBC and WCR performed sequence and bioinformatics analysis. WCR analyzed and interpreted the data, and drafted the article. All authors provide editorial content and have read and approved the final manuscript. “”The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age disability, and where applicable, sex, marital status, famial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual’s income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who required alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA’s TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD).