Satellite cells could excrete growth factors including VEGF that would induce angiogenesis and improve cell survival (14). The VEGF is the prototypic member of a family of secreted, homodimeric glycoproteins with endothelial cell-specific mitogenic activity and the ability to stimulate angiogenesis in vivo (15). On the other hand, a number of growth factors such as fibroblast

Inhibitors,research,lifescience,medical growth factor (FGF) can promote the activation and proliferation of skeletal satellite cell (16, 17). TNF-α is an early and potent pro-inflammatory cytokine that stimulates the inflammatory response. Even minor trauma to Selleckchem Carboplatin muscle will increase levels of TNF-α by release from mast cells. It is also produced by neutrophils, macrophages and lymphocytes that accumulate rapidly at the site of injury. TNF-α increases rapidly within damaged myofibers and is expressed Inhibitors,research,lifescience,medical by myoblasts and myotubes (18–20). It is greatly elevated in injured normal damaged myofibers (18,

19) and myopathic skeletal muscle (21); is chemotactic for Inhibitors,research,lifescience,medical myoblasts in vitro (22) and mitogenic for satellite cells in vivo (20), suggesting a direct role in myogenesis of regenerating muscle (18). The apoptosis cascade can be triggered by 2 main pathways, via an intrinsic, endogenous system such as the mitochondrial Bax/Bcl-2 or via an extrinsic system Fas and FasL involving transmembrane receptors of the death receptor family (23). FasL ligand induces Inhibitors,research,lifescience,medical apoptosis

through cognate interaction with its receptor Fas (24). FasL is mainly present in activated T lymphocytes, natural killer cells, and macrophages (25, 26). The aim of the present study is to investigate markers of degeneration and regeneration in blood of DMD patients Inhibitors,research,lifescience,medical compared to controls. Markers of degeneration are measured in terms of increased Fas/FasL and Bax/Bcl-2 and plasma DNA fragmentation. Markers of regeneration are the cytokine TNF-α and the growth factors: VEGF and bFGF. Subjects and methods Subjects were 24 boys diagnosed clinically and at the molecular level as having DMD (mean of age (8.1 ± 1.9), versus 20 age and socioeconomic matching healthy boys (mean of age 8.2 ± 2.2). Patients and controls were chosen 17-DMAG (Alvespimycin) HCl to be free from any infection and receiving no therapeutic treatment known to increase the oxidative stress. Blood samples were drawn after their parents’ consent. Methods Reverse Transcriptase-polymerase Chain Reaction (RT-PCR) Analysis for FAS-Ligand and Bax Total RNA was extracted from lymphocytes using QIAGEN RNA extraction kit (QIAGEN Inc, USA). The RNA samples were reverse transcribed using superscript reverse transcriptase, using QIAGEN OneStep RT-PCR kit (QIAGEN Inc USA, Clini Lab).