0498 (ANOVA; P = 0.0000). The alterations in the bone marrow cell type composition selleck chemicals llc of mice from the same experiment are presented in this website Figure 4. The infection of control mice (CP-P-B+ versus CP-P-B-) led to an increase of the segments content (P = 0.0001) and co-administration
of phages (CP-P+B+ group) markedly increased the percentage of myelocytes (P = 0.0016) and metamyelocytes (P = 0.0000). In CP-treated and infected mice (CP+P-B+) there was a deficit of bands and no segments were present, however application of phages in these mice (CP+P+B+ group) led to a significant (a two-fold) mobilization of myelocytes (P = 0.0068) and bands (P = 0.0495). Interestingly, the phages alone (CP-P+B-) increased (P = 0.0000) the content of segments in control, not infected mice (CP-P-B-). Other changes following phage administration were not significant. Figure 4 Effects of A5/L phages on the bone marrow cell composition in cyclophosphamide-treated and S. aureus -infected mice. S – segments, B – bands, Me – metamyelocytes, My – myelocytes, O – other. Mice were given CP
(350 mg/kg b.w.). After four days 1 × 106 A5/L phages and 5 × 106 S. aureus were administered. The bone marrow was isolated on day 0, just before administration of CP (Control) and at 24 h following infection (day click here 5). The results are presented as the mean value of 5 mice per group. Statistics (day 5): Segments: CP-P-B+ vs CP+P-B+ P = 0.0001 (ANOVA; P = 0.0000); Bands: CP-P-B+ vs CP+P-B+ P = 0.0009; CP+P-B+ vs CP+P+B+ P = 0.0495 (ANOVA; P = 0.0000); Metamyelocytes: CP-P-B+ vs CP-P+B+ P = 0.0003 (ANOVA; find more P = 0.0000); Myelocytes: CP+P-B+ vs CP+P+B+ P = 0.0062 (ANOVA; P = 0.0000); Other: CP-P-B+ vs CP+P-B+ P = 0.0003 (ANOVA;P = 0.0000). Statistics (day 0 vs day 5): Segments: CP-P-B- vs CP-P-B+ P = 0.0001; CP-P-B- vs CP-P+B- P = 0.0000 (ANOVA); Metamyelocytes:
CP-P-B- vs CP-P+B+ P = 0.0000 (ANOVA); Myelocytes: CP-P-B- vs CP-P+B+ P = 0.0016 (ANOVA). Effects of the phages on generation of the humoral response to S. aureus and to sheep erythrocytes A possibility existed that phages, beside their direct, protective role during infection, may stimulate generation of specific immune response against bacteria. Figure 5 shows the effects of phage administration on the agglutinin level in mouse sera taken 21 days following intraperitoneal immunization of mice with 5 × 106 of S. aureus (for details see Materials and Methods). The results revealed a strong up-regulation (P = 0.0001) of anti-S. aureus agglutinin titer in CP and phage-treated mice (CP+P+B+) in comparison with a respective control (CP-treated mice) (CP+P-B+ group). The analogous effect of phages in mice not treated with CP was minor (CP-P+B+ versus CP-P-B+ group). The phages also enhanced (not significantly), the titer of hemagglutinins to SRBC in CP-treated and infected mice (data not shown).