The effect of formulation variables such as molecular weight of outer rupturable coating layer, type and amount of swelling layer, weight gain of coating film and influence of paddle speed on drug release were investigated. Adriamycin supplier The drug release from the pulsatile tablets exhibited an initial lag period, followed by a stage of rapid drug release. The optimal level of swelling layer (crospovidone) to achieve a fast and complete release was 20 % w/w. The results indicated that lag time was prolonged with the increased weight gain of coating film. Also no significant

difference in the drug release was observed for different rotational speeds. In accordance with the chronomodulated therapy of asthma, the lag time criterion of 5 hours was satisfied by formulation having 11 % weight gain of outer rupturable layer.”
“A simple and cost-effective method by capillary zone electrophoresis (CZE) was developed and validated for the simultaneous determination of the antituberculosis drugs isoniazid (INH), pyrazinamide (PYR), and rifampicin (RIF) in tablets. A 40 mmol L(-1) sodium

tetraborate background electrolyte (BGE) solution (pH 9.0) was found to be suitable for separation of all the analytes. An uncoated fused-silica capillary of 643 cm length (effective length 56 cm) was used for chromatography separation. All analytes were completely separated within 5 min at an applied voltage of 20 kV (max. 50 AZD1208 clinical trial mu A), and detection was performed at 269.5 nm with APR-246 an UV detector. The method was validated in terms of linearity, accuracy, precision, and robustness. The linearities of the calibration curves for INH, PYR, and RIF were 40-120 mu g/mL (r(2) = 0.9994), 20-100 mu g/mL (r(2) = 0.9997), and 40-100 mu g/mL (r(2) = 0.9999), respectively. The proposed method was successfully applied for the simultaneous determination of the tuberculostatic drugs RIF, INH, and PYR in tablets. Thus, the proposed CZE method is a potential alternative to the HPLC methods described by the US Pharmacopoeia for the quality control of tuberculostatic drugs.”
“Metronidazole (MTZ) is a wonderful drug, which

is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to evaluate the possible cell death mechanism associated with it. A significant decrease in Mitotic Index (P < 0.001) as well as an increase in Sister Chromatid Exchange (P < 0.001) and Chromosomal Aberrations (P < 0.001) frequencies with no modifications in Replication Index was observed. DNA extracts of MTZ treated cells resulted in nucleosomal DNA ladder pattern after 48 h of cell treatment and this pattern correlated with a decrease in cellular viability (P < 0.