The obtained data comple ments and expands the known spectrum of immunity re lated genes and provides a valuable platform for more detailed analyses of the immune response in fish in general a turbot in particular. Several immune related pathways were also identified in the Turbot 3 database. Chemokine signaling is an important immune pathway due to the fundamental Inhibitors,Modulators,Libraries role of Inhibitors,Modulators,Libraries chemokines in providing directional cues for the trafficking of leukocytes to sites of inflammation but also it has been implicated in dendritic cell maturation, macrophage activation, neutrophil degranulation, B cell antibody class switching, and T cell activation. The data infers that chemokines influence both the innate and ac quired phase of an immune response to host insults.

Thus, the protein richness of this pathway in the Turbot 3 database was described. Most members intervening in this pathway were identified showing the usefulness of the Turbot 3 database for gene discovery. Identification of genes related Anacetrapib to reproduction Inhibitors,Modulators,Libraries To date, fish gonad related ESTs are poorly represented in public databases. A first attempt to identify genes re lated to gonad development in male and Inhibitors,Modulators,Libraries female turbot was carried out by cDNA AFLP technology and several specific sequences could be identified. However, the amount of information presently available is still scarce and thus a small number of sex specific sequences have been identified. Here, the use of the 454 FLX Titanium sequencing allowed obtaining a large number of gene se quences and their subsequent assembly and gene annotation led to the identification of a total of 1,410 annotated sequences related to reproductive func tion. This means that sequences corresponding to many genes of the brain hypophysis gonad axis, expressed first during the process of sex differentiation and then during gonadal maturation, have been identified. Functional annotation terms classified all those se quences in a total of 8,425 GO terms.