Therefore, we speculate that chromosome/nucleosome process activities are strongly affected by AvrA at 8 hours post infection by SL1344. Figure 4 Graphical output of Multi-GOEAST cellular component analysis results for genes differentially expressed by SL1344 and SB1117

infected mouse colon at 8 hours. Red Boxes represent enriched GO terms only found in up-regulated genes in the SL1344 vs SB1117 infection groups, and green boxes represent enriched GO terms only found in down-regulated genes in SB1344 vs SB1117 infection groups. The saturation degrees of all colors represent the significance of enrichment selleck screening library for corresponding GO terms. Arrows represent connections between different GO terms. Red arrows represent relationships between two enriched GO terms, black solid arrows represent relationships between enriched Buparlisib research buy and unenriched terms and black dashed arrows represent relationships between two unenriched GO terms. In Table 3, 268 genes were up-regulated

in the SL1344 vs SB1117 infection groups at 4 days. Among them, 134 transcripts were assigned specific GO terms. A significant number of transcripts were assigned known functions in biological regulation (70 genes), regulation of cellular process (67 genes), multicellular organismal process (47 genes), signal transduction (45 genes) and apoptosis (10 genes). An interesting result was that a total of 25 differentially expressed olfactory receptor family members participated in all of the biological processes except for apoptosis (Table 3). In the SL1344 vs SB1117 infection group at 4 days, 337 genes were down-regulated Adenosine genes (Table 4). Of these gene, 201 transcripts were assigned specific GO terms, and a significant number of transcripts were assigned known functions in system process regulation (39 genes), neurological system processes (37 genes), and G protein-coupled receptor protein signaling pathway (35 genes). These biological processes may selleck kinase inhibitor underlie the physiological deficits of bacterial infection by inducing a decline in gene transcription. The ontology of the cellular component for down-regulated and up-regulated genes showed that most of molecular activity occurred in the cell

membrane at 4 days post infection (data not shown). AvrA targeted specific pathway and network analysis An over-representation of a specific biological process does not indicate whether the process in question is being stimulated or repressed overall. We used IPA software to further investigate over- or under-represented functional activities of AvrA, specifically within the up-regulated and down-regulated genes, at the stage of infection at 8 hours and 4 days. We focused on the ingenuity canonical pathways and addressed the differentially up-regulated genes between the SL1344 vs SB1117 infection groups at 8 hours and 4 days post infection (Table 5 and Table 6). Table 5 Target pathway of up-regulated Genes in SL1344 vs SB1117 infection groups at 8 hours.