(TIFF 901 KB) Additional file 2: Figure S2: Conjugation scheme. Typhimurium ST213 strain YU39 was used as donor of the bla CMY-2, gene (conferring resistance to ceftriaxone; CRO) carried by the pA/C plasmid. Five recipient strains were tested:
two Typhimurium ST19 strains (SO1 pSTV::Km and LT2 pSTV::Km), and three E. coli strains (DH5α, HB101 and HB101pSTV::Km). The relevant plasmids are depicted by dotted circles (see text for details). (TIFF 2 MB) Additional file 3: Table S1: Primers used in this study. (DOC 68 KB) Additional file 4: Figure S3: PCR typing scheme for pX1. The six regions used in the pX1 typing scheme are Ilomastat show on the sequence of the plasmid (unpublished data). The regions involved in plasmid replication oriX and ydgA are in blue; the regions involved in conjugation taxB, taxC and ddp3 are in red; the intergenic region between 046-047
hypothetical protein genes and the stbDE operon were the CMY island was inserted (Figure 1) are in green. (TIFF 2 MB) References 1. Zaidi MB, Calva JJ, Estrada-Garcia MT, Leon V, Vazquez G, Figueroa G, Lopez E, Contreras J, Abbott J, Zhao S, et al.: Integrated food chain surveillance system for Salmonella spp. in Mexico. Emerg Infect Dis 2008, 14:429–435.PubMedCrossRef Talazoparib 2. Zaidi MB, Leon V, Canche C, Perez C, Zhao S, Hubert SK, Abbott J, Blickenstaff K, McDermott PF: Rapid and widespread dissemination of multidrug-resistant blaCMY-2 Salmonella Typhimurium in Mexico. J Antimicrob Chemother 2007, 60:398–401.PubMedCrossRef 3. Silva C, Wiesner M, Calva E: The Importance of Mobile Genetic VS-4718 mouse Elements in the Evolution of Salmonella : Pathogenesis, Antibiotic Resistance and Host Adaptation. In Salmonella
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