5–4.5 Gy (dose). Figure 2M–P presents the minimum growth rate (27–58%) for 42 h obtained using the irradiation parameters, 90 MeV u-1 (energy), 60–120 keV μm-1 (LET), and 1.5–4.5 Gy (dose). These data suggest that the cellular growth rate of the D. natronolimnaea svgcc1.2736 strain is dependent on the irradiation energy of the 12C6+ions. Significant differences in the effects of 12C6+ ions at the same doses were also observed. This suggests a strong dependence of low-dose effects on LET (Figure 2I-L). Figure 2 12 C 6+ -ions see more of different parameters irradiation level and

its effect on the growth rate of D. natronolimnaea smgcc1.2736 strains cells in %. (A-D) 12C6+-ions were accelerated up to 30 MeV/u, and their LETs were 60, 80, 100 and 120 keV/μm, with a dose rate of 0.5-1.5Gy. (E-H) 12C6+-ions were accelerated up to 45 MeV/u, and their LETs were 60, 80, 100 and 120 keV/μm, with a dose rate of 0.5-1.5Gy. (I-L) 12C6+-ions were accelerated up to 60 MeV/u, and their LETs were 60, 80, 100 and 120 keV/μm, with a dose rate of 0.5-1.5 Gy. (M-P) 12C6+-ions were accelerated up to

90 MeV/u, and their LETs were 60, 80, 100 and 120 keV/μm, with a dose rate of 0.5-1.5 Gy. Effect of irradiation dose on productivity of D. natronolimnaea svgcc1.2736 Different irradiation doses showed a notable affect on the growth rate and conidia aggregation in D. natronolimnaea svgcc1.2736. CX production in 1 L cultures of D. natronolimnaea svgcc1.2736 mutants was, shown to be sensitive to irradiation dose

(Figure 3). XAV-939 manufacturer Overall, for CX producing strains of D. natronolimnaea svgcc1.2736 mutants, increasing the irradiation dose from the standard 0.5 to 4.5 Sepantronium concentration Gy led to a considerable much decline in dry cell weight (BDW), from around 8.71 ±0.04 to 2.23 ±0.06 g L-1, respectively. The CX yield, however, showed an almost two-fold increase from 8 ±0.9 to 12 ±0.2 mg L-1. To find the optimal 12C6+ irradiation dose for the process, a considerable amount of cell culture was carried out using similar irradiation experiments. natronolimnaea svgcc1.2736 strains productivity increases by almost six-fold. Optimal production of 0.81 mg L-1 h-1 was detected at a irradiation dose of approximately 4.5 Gy at an 80 keV μm-1 LET and 60 MeV u-1 energy level (Figure 3B). In contrast, 12C6+ irradiation with a LET of more than 100 keV μm-1, and energy level of greater than 45 MeV u-1 reduced the rate of production (Figure 3D). 12C6+ irradiation with LET (80 keV μm-1), energy (60 MeV u-1) and dose (1.5 Gy) led to perfect mycelial growth (Figure 3A). The increased irradiation dose of 12C6+ however led to a decrease in biomass in this strain (Figure 3). Figure 3B depicts the BDW and productivity of the strains with respect to different energy (45 and 60 MeV u-1) versus an irradiation dose with a LET of 80 keV μm-1.