Anal Bioanal Chem 2007, 387:83–89.PubMedCrossRef 20. Hansmeier N, Albersmeier A, Tauch A, Damberg T, Ros R, Anselmetti D, Pühler A, Kalinowski J: The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032. Microbiology 2006, 152:923–935.PubMedCrossRef 21. Hansmeier N, Bartels FW, Ros R, Anselmetti D, Tauch A, Pühler A, Kalinowski J: Classification of hyper-variable Angiogenesis inhibitor Corynebacterium glutamicum surface layer proteins by sequence analyses and atomic force microscopy. J Biotechnol 2004, 112:117–193.CrossRef 22. Tsuge

Y, Ogino H, Teramoto H, Inui M, Yukawa H: Deletion of

cg_1596 and cg_ encoding NlpC/P60 proteins, causes a defect in cell separation in Corynebacterium glutamicum R. J Bacteriol 2070, 190:8204–8214.CrossRef 23. Watt SA, Wilke A, Patschkowski T, Niehaus K: Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100. Proteomics 2005, 5:153–167.PubMedCrossRef 24. Schägger H, von Jagow G: Tricine-sodium dodecyl sulfate-polyacrylamide gel eletrophoresis for the separation of proteins in the range LB-100 chemical structure from 1 to 100 kDa. Anal Biochem 1987, 166:368–379.PubMedCrossRef 25. Blum M, Beier H, Gross HJ: Improved silverstaining of plant proteins, RNA and DNA in polyacrylamid gels. Electrophoresis 1987, 8:93–99.CrossRef 26. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. 2nd edition. Cold Spring Habor Laboratory Press Cold Spring Habor, NY; 1989. 27. Schäfer A, Tauch A, Jäger W, Kalinowski J, Thierbach G, Pühler A: Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: Selection of defined deletions in the chromosome of Corynebacterium glutamicum . Gene 1994, 145:69–73.PubMedCrossRef Galeterone 28. Grant SGN, Jessee J, Bloom FR, Hanahan D: Differential selleck chemical plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci USA 1990, 87:4645–4649.PubMedCrossRef

29. Peterson WD Jr, Stulberg CS, Swanborg NK, Robinson AR: Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc Soc Exp Biol Med 1968, 128:772–776.PubMed Authors’ contributions LO carried out growth mutagenesis experiments, invasion assays, fluorescence microscopy, protein preparation and analysis, MHö carried out adhesion experiments, RGG and MHe supported LO and MHö in respect to cell culture, adhesion and invasion analysis and fluorescence microscopy, AFM experiments were carried out in cooperation with JR and TES, AB supervised the experiments of LO and MHö and was responsible for the draft and final version of the manuscript. All authors read and approved the final manuscript.