P -value Host species 2/1 RD = 0.7 ± 14.6, FD = -15.0 ± 17.4 0.99 Area 4/1 CR = 8.2 ± 37.9, EB = 0.4 ± 2.3, MA = -10.4 ± 28.8, PU = 0.99 ± 2.0

0.96 Age 1/85 0.8 ± 0.7 0.24 Distance to marsh 1/78 2.7 ± 2.9 0.03 Distance to other host species similarly infected 1/94 -1.3 ± 0.4 0.19 Host species*area 2/74 Not shown 0.53 Host species*Distance to marsh 7/1 RD*distance = 0.5 ± 4.5, FD*distance = 6.3 ± 5.7 0.96 Distance to other host sim. inf. *host species 2/95 RD*distance = 2.2 ± 1.2, FD*distance = 3.8 ± 1.1 0.002 (ii) M. bovis A1 Host species 2/103 RD = -0.8 ± 1.2, FD JNK-IN-8 datasheet = -2.1 ± 1.1 0.18 Area 4/97 EB = -0.9 ± 1.2, MA = -3.0 ± 1.5, PU = -2.8 ± 1.2 0.008 Distance to marsh 1/97 -1.7 ± 1.3 0.20 Distance to other host species similarly infected 1/111 0.1 ± 0.2 0.81 (iii) M. scrofulaceum Host species 2/87 RD = 2.4 ± 1.8, FD = 6.3 ± 1.7 0.001 Area 4/85 CR = -5.4 ± 1.9, EB = -1.2 ± 1.7, Pictilisib purchase MA = -9.8 ± 13.0, PU = -2.0 ± 2.3 0.08 Distance to marsh 1/72 2.1 ± 1.9 0.26 Distance to other host

species similarly infected 1/119 0.8 ± 0.4 0.03 Reference levels for ‘Area’ and ‘Host species’ are ‘SO (Sotos)’ and ‘wild boar’ respectively. FD = fallow deer, RD = red deer. CR = Coto del Rey, EB = Estación Biológica, MA = Marismillas, PU = El puntal. Statistics concerning the GLMMs to test the factors affecting the presence of a given mycobacterial type or group are shown in Table 9. Concerning the M. bovis

vs MOTT GLMM, the distance to water was statistically higher in MOTT infected individuals than in M. bovis ones (MOTT mean distance to water = 1989 ± 245 m; M. bovis mean distance to water ± SD = 1513 ± 164 m). The ratio of the minimum distances to similarly infected hosts (which in average were Wortmannin mw always higher than 1 for the three host species and analyzed mycobacterial groups) statistically interacted with the host. The ratios (log10-trasnformed) were similar for MOTT and M. bovis in both Reverse transcriptase deer species (2.13 ± 0.36 and 2.11 ± 0.32 for MOTT and M. bovis in red deer; 2.01 ± 0.11 and 1.95 ± 0.35 m for MOTT and M. bovis in fallow deer), whereas they were higher for M. bovis than MOTT in the wild boar (2.71 ± 0.36 and 3.55 ± 0.20 m for MOTT and M. bovis). This would indicate that in wild boar the intraspecific spatial aggregation of M. bovis is higher than for MOTT. When attending to specific mycobacterial types, there were statistical differences between zones for bovis TP A1, so that it was dominant in wild ungulates from the north of DNP (Table 1, Figure 6). There were statistical differences in the probability of infection by M. scrofulaceum relative to other types among host species (wild boar = 7.3%; Red deer = 18.2% and fallow deer = 41.0%; Table 1). M. scrofulaceum presented a lower intraspecific spatial aggregation than the remaining mycobacterial types (2.19 ± 0.