Fat metabolism is an important indi cator of how efficiently and to what extent these sellekchem factors are competently integrating. Obesity is a condition in which adipocytes accumulate a large amount of fat and become enlarged. It is characterized at the cellular level by an increase in the number and size of adipocytes differen tiated from fibroblastic preadipocytes in adipose tissue. The adipocyte is the primary site for energy storage, which accumulates triglycerides due to factors that include nutri tional excess, nutrient deficiencies, excessive stress, and genetic predispositions among other causes. Shimomura et al. indicated that adipocytes synthesize and secrete biologically active molecules called adipocytokines.

During adipocyte differentiation, tran scriptional factors such as peroxisome proliferator acti vated receptor gamma are involved in the sequential expression of adipocyte specific proteins. Adiponectin is an adipocytokine that has been shown to have antiatherogenic, anti inflammatory, and antidia betic roles. It has been found to be an important mod ulator of insulin sensitivity. Nakamura et al. indicated that high circulating levels of adiponectin might be protective against the development of coronary artery disease. Adiponectin levels are inversely correlated to body fat percentage, indicating that adiponectin plays an important role in fatty acid catabolism. Yamauchi et al. indicated that adiponectin has emerged most recently as an important adipocytokine with insulin sensitizing effects and represents a novel treatment target for insulin resistance and type 2 diabetes.

Leptin is a secreted protein hormone that affects the hypothalamus to inhibit food intake and stimulates thermogenesis. The cytosolic enzyme Glycerol 3 Phosphate Dehydrogenase appears to have an important role catalyzing the conver sion of glycerol into triglyceride. In the present study, we investigated the effects of a pro prietary extract of OB131 Irvingia gabonensis on the inhibition of intracellular triglyceride and G3PDH activity in 3T3 L1 adipocytes. We also examined the effect of these compounds on protein expression of adipogene sis in 3T3 L1 adipocytes. Methods Cell Culture A murine 3T3 L1 cell line was used in this study due to its widespread acceptance as a cell model for adipose cell biology research over the course of several decades.

3T3 L1 preadipocytes were purchased from the Bioresource Collection and Research Center. 3T3 L1 preadipocytes were planted into 6 well plates and maintained in DMEM sup plemented with 10% bovine calf serum at 37 C in a humidified 5% CO2 incubator. Adipocytic differentiation was induced by the adipogenic agents that were added to culture medium. Afterwards, the medium was changed to normal Cilengitide culture medium and was freshly replaced every 48 h.