The membranes were blocked, washed and incubated with specific primary antibodies. The primary antibody incubation was fol lowed by incubation with HRP conjugated secondary antibodies. The bands were detected with an enhanced chemiluminescence assay. ELISA Various ovarian cancer www.selleckchem.com/products/Erlotinib-Hydrochloride.html cell lines were seeded in 60 mm plates and incubated with Corilagin or DMSO. Culture supernatants were harvested after 1, 2, and 3 days to measure the concen tration of TGF B1. Hey cells were seeded in 96 well plates and incubated with Corilagin, Paclitaxel, or DMSO the next day. Culture supernatants were harvested at 48 h to measure the concentration of TGF B1. SRB was used to detect the effects of Corilagin and Paclitaxel on the proliferation of ovarian cancer cells.

The concentration of TGF B1 was measured by ELISA according to the manufacturers instructions. mice. The SKOv3ip cells were injected subcutaneously. Tumors were measured twice a week, and tumor volumes were calculated using the formula TV /2, where L represents the longer diameter and W represents the shorter diam eter. When palpable tumors had grown to a diameter of 0. 3 0. 5 cm, the mice were divided into four groups of six to eight, and each group received an intraperi toneal injection of either DMSO or 5, 10, or 15 mg/kg of Corilagin. The doses of Corilagin Growth of xenografts in nu/nu mice All animal experiments were carried out in accor dance with an animal protocol approved by the Insti tutional Animal Care and Use Committee of the Shanghai Tumor Institute.

The effect Cilengitide of Corilagin on the in vivo growth of ovarian cancer xenograft tumors was evaluated using xenografts of the human ovarian cancer cell line SKOv3ip in Balb/c nu/nu used were in reference to the animal experiments of Hau DKs group. The mice were treated three times per week for four weeks and were then sacrificed. Statistical analysis All data were subjected to statistical analysis and were reported as the mean standard deviation. The criterion for statistical significance was taken as P 0. 05 using a two tailed t test and the count data were tested using chi square criterion comparing the parameters frequency of parameters. The analyses were performed using SPSS 15. 0 software. Results Corilagin inhibits the growth of ovarian cancer cell lines in vitro and in vivo Ovarian cancer cell lines and normal OSE cells were used to examine the effects of Corilagin in cell culture. Corilagin demonstrated clear inhibition of ovarian cancer cell growth but had much lower cytotoxicity in normal OSE cells, with IC50s of approximately 160 uM. To determine if Corilagin had the same effect in vivo, Corilagin was delivered by intraperitoneal injection into mice bearing SKOv3ip xenografts.