The nine species common to both consortia had similar sequential development on cheese surface. Lc. lactis, used as starter MM-102 research buy culture for cheese manufacture, was part of the dominant flora until day 7 and disappeared thereafter. St. equorum was the first species to colonize the surface within 7 days. Al. kapii grew on day 14 concomitant with C. casei and B. linens, followed by C. variabile, an uncultured bacterium from marine sediment and Mc. gubbeenense between day 14 and 37. Agrococcus casei was first detected on day 37. Other

species specific to consortium F (St. vitulinus, Enterococcus sp., M. psychrotolerans, Brachybacterium sp. and Br. tyrofermentans) colonized the corresponding cheese after 7 to 21 days ripening.

Both Brachybacterium species also colonized the cheese treated with consortium M, but could only be detected after 81 days, together with the Brachybacterium species specific to consortium M (Br. paraconglomeratum). Repetition of both treatments revealed the same trends with minor differences including a growth delay (ca. 5 days) for some high-GC species and the additional development of M. psychrotolerans at day 20 on selleck chemicals llc the cheese treated with consortium M (data not shown). Table 3 Population dynamics of cheese surface consortia by TTGE1 Bacterial species detected with TTGE   Band designation2   Consortium F (ripening day)   Consortium M (ripening day)   OMK 704 (ripening day)     1 7 14 21 37 81   1 7 14 21 37 81   1 7 14 21 37 81 Ag. Casei   x           + d.           + d.               Al. kapii   y   d.   + d. d.     d.   + d. d.             +   Br. paraconglomeratum   m                 d.         +               Brachybacterium sp., or A. arilaitensis   l   d. d. + d. d. d.             +       + d. d. d. Br. tyrofermentans   k   d.     + d. d.             +             + B. linens   a, e, g, h, i, n, o

  d. d. + d. d. d.   d. d. + d. d. d.     + d. d. d. d. C. casei   h, j, v   d. d. + d. d. d.   d. d. + d. d. d.               C. variabile   b, c   d. d.   + d. d.   d. d. + d. d. d.   d. + d. d. d. d. E. malodoratus   r       + d.                                 Lc. CH5424802 purchase lactis   w (without z’)   d. d.           d. d.           d. d.       Etomidate   M. psychrotolerans   w and z’       + d.                             +   Mc. gubbeenense   d   d.     + d. d.           + d.               St. equorum, St. epidermidis, or F. tabacinasalis   q   d. + d. d. d. d.   d. + d. d. d. d.       + d. d.   St. equorum   q and t   d. + d. d.       d. + d. d.           + d.     St. vitulinus   p   d. + d. d.                         + d. d.   uncultured bacterium from marine sediment   f   d. d.   + d.     d. d.     + d.             + 1 The letter (d.) indicates sampling times where a given species was detected in the TTGE gel. The symbol (+) indicates growth of a species in the smear. Growth was assumed in two cases, i.e.