This approach eliminated the need to demonstrate long-term nanoparticle storage stability and, owing to a

single mixing step, permitted a facile preparation protocol to which it was easy for personnel at animal facilities and hospital/clinic pharmacies to adhere. 6. RONDEL Proof of Concept in Tumor-Bearing Mice: Expanded Nanoparticle Characterization Having developed small-scale synthetic procedures for the three aforementioned components of the delivery system (CAL101, AD-PEG, and AD-PEG-Tf), an appropriate in vivo model was Inhibitors,research,lifescience,medical sought for a proof-of-concept investigation of the ability of this system to deliver siRNA to tumor cells in Inhibitors,research,lifescience,medical mice. In collaboration with Dr. Timothy Triche and colleagues at Children’s Hospital Los Angeles, a disseminated murine model of Ewing’s family of tumors (EFT)—mesenchymal malignancies that arise in bone or soft tissue or present as primitive Tyrphostin AG-1478 research buy neuroendocrine tumors and typically affect teenagers—was identified and selected. The vast majority (85%) of EFT patients have a

unique chromosomal translocation that results in the creation of a chimeric EWS-Fli1 fusion that serves as an oncogenic transcription factor. Accordingly, Inhibitors,research,lifescience,medical siRNA species targeted specifically to the region of fusion had been described [32] which could induce apoptosis of EFT cells. A potent published anti-EWS-Fli1 siRNA was utilized within Tf-targeted nanoparticles to investigate the effect of treatment on cumulative tumor burden in mice. To create a

disseminated EFT model in mice Inhibitors,research,lifescience,medical Inhibitors,research,lifescience,medical for which tumor burden could be readily measured, systemic (tail vein) injections were made of EFT cells which constitutively expressed firefly luciferase; this allowed the use of whole-animal bioluminescence imaging to quantify tumor burden. Employing a twice-weekly dosing regimen for four weeks, a statistically significant reduction in tumor burden was observed only for those nanoparticles which contained (i) the anti-EWS-Fli1 siRNA and (ii) the Tf targeting ligand (Figure 9(a)). Importantly, this was achieved in the absence of strong indications of toxicity or immunogenicity in these animals (Figure 9(b)). Together, these findings suggested Cytidine deaminase a strong potential for continued development of this platform of siRNA-containing nanoparticles as anticancer therapeutics. Figure 9 RONDEL-based nanoparticles containing siRNA against EWS/Fli-1 were well tolerated by mice and efficacious in a disseminated murine model of Ewing’s sarcoma. (a) When administered twice weekly for four weeks, only nanoparticles containing AD-PEG-Tf and …