pneumophila alone or together with Pseudomonas aeruginosa Lastly

pneumophila alone or together with Pseudomonas aeruginosa. Lastly, the inter-laboratory comparison revealed that the ScanVIT test exhibits a lower variability than the traditional culture

test (mean coefficient of variation 8 center dot 7 vs 16 center dot 1%).

Conclusions:

The results confirmed that the ScanVIT largely overlaps the reference method and offers advantages in terms of sensitivity, quantitative reliability and reduced assay time.

Significance and Impact of the Study:

The proposed method may represent a useful validated alternative Temsirolimus order to traditional culture for the rapid detection and quantification of Legionella spp. in water.”
“Aims:

Single-walled carbon nanotubes (SWNTs) are likely to become increasingly widespread and yet their environmental impact is not well understood. The purpose of the current study

was to evaluate the impact of SWNTs on microbial communities in a ‘sentinel’ environmental system, activated sludge batch-scale reactors.

Methods and Results:

Triplicate batch reactors were exposed to SWNTs and compared to control reactors exposed to impurities associated with SWNTs. Automated ribosomal intergenic spacer analysis (ARISA) was used to assess bacterial community structure in each reactor. SWNT exposure was found to impact microbial community structure, while SWNT-associated impurities had no effect, compared to controls. 16S rRNA gene sequence analysis indicated that dominant phylotypes detected by ARISA included members of the families Sphingomonadaceae and Apoptosis inhibitor Cytophagacaceae and the genus Zoogloea. Ureohydrolase ARISA results indicated an adverse impact of SWNTs on the sphingomonad relative to other community members. Changes in community structure also occurred in both SWNT-exposed and control reactors over the experimental time period and with the date on which activated sludge was obtained from a wastewater treatment facility.

Conclusions:

These results indicate that SWNTs differentially

impact members of the activated sludge reactor bacterial community.

Significance and Impact of the Study:

The finding that community structure was affected by SWNTs indicates that this emerging contaminant differentially impacted members of the activated sludge bacterial community and raises the concern that SWNTs may also affect the services it provides.”
“Aims:

To characterize the bacterial communities in commercial total mixed ration (TMR) silage, which is known to have a long bunk life after silo opening.

Methods and Results:

Samples were collected from four factories that produce TMR silage according to their own recipes. Three factories were sampled three times at 1-month intervals during the summer to characterize the differences between factories; one factory was sampled 12 times, three samples each during the summer, autumn, winter and spring, to determine seasonal changes. Bacterial communities were determined by culture-independent denaturing gradient gel electrophoresis.

Comments are closed.