These components of the host lysis cassette are every current from the genome of phages eiAU, eiDWF, and eiMSLS together with a putative Rz lysis accessory protein encoded by ORF38. The RZ protein is predicted to be a kind II integral membrane protein and its perform, despite the fact that not fully understood, may be expected for host cell lysis only in the medium con taining an excess of divalent cations. Phage endoly sins have already been linked to five enzymatic pursuits, which include an N acetyl muramidase or real lysosyme, the lytic transglycosylases, the N acetylmuramoyl L ala 9 amidases, the endo b N acetylglucosaminidases, plus the endopeptidases. Secondary construction evaluation predicts the endolysin of eiAU is a member on the N acetylmuramoyl L alanine amidases class of endolysins.
DNA replication proteins ORFs with important sequence similarity to proteins concerned in DNA replication have been recognized in all 3 E. ictaluri specific phage genomes. ORF44 is predicted to encode a phage replicative helicase primease. info Many phages use separate primase and helicase proteins whilst other people use a multifunctional protein possessing the two routines. The helicase primase pro tein will work in DNA replication by unwinding double stranded DNA into single stranded DNA. No pre dicted perform can be assigned to ORFs45 and 46. Also, no predicted function may very well be assigned to ORF47. how ever, a hunt for secondary structures inside the pre dicted ORF47 amino acid sequence detected a helix hairpin helix DNA binding motif. Moreover, no puta tive function might be assigned to ORF48, ORF49, or ORF50.
ORF51 had as among its top BLAST hits an iso prenylcysteine carboxyl methyltransferase recognized to func tion in methylating isoprenylated amino acids. ORF52 is predicted to encode a protein just like gp41 of Sodalis phage SO 1, but no putative perform could be assigned. ORF53 is predicted to encode DNA polymerase I. 2nd ary framework evaluation SRC Inhibitors molecular advised the DNA polymerase encoded by ORF53 includes a domain that is responsible for your 3 five exonuclease evidence reading through activity of E. coli DNA polymerase I as well as other enzymes, and catalyses the hydrolysis of unpaired or mismatched nucleotides. The protein encoded by ORF54 is predicted to possess a VUR NUC domain, that are connected with members of the PD XK nuclease superfamily such as type III restric tion modification enzymes.
ORF2 is predicted to encode a DNA repair ATPase. A look for secondary structures inside of the ORF2 predicted amino acid sequence uncovered a HNH endonuclease. No putative function could be assigned to ORF3. ORF4 is predicted to encode a helicase protein belonging to the SNF2 family, commonly observed in proteins concerned in the number of processes together with tran scription regulation, DNA restore, DNA recombination, and chromatin unwinding. ORF6 is predicted to encode a phage methyltransferase. Secondary framework analysis revealed the methyltransferase predicted to become encoded by ORF6 is usually a C five cytosine particular DNA methylase which in bacteria is really a part of restriction modification methods. Also, Mg and ATP binding sites have been detected within the predicted protein solution of ORF6. ORF7 is predicted to encode a DNA N 6 adenine methyl transferase within a relatives of methyltransferase located in bacteria and phage that has internet site precise DNA methyltrans ferase activity. No ORF encoding an RNA polymerase was detected in any with the phages suggesting that these phages rely on the host RNA polymerase to transcribe their genes.